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Effects of decellularization on the mechanical and structural properties of the porcine aortic valve leaflet

Paper ID Volume ID Publish Year Pages File Format Full-Text
11257 727 2008 10 PDF Available
Title
Effects of decellularization on the mechanical and structural properties of the porcine aortic valve leaflet
Abstract

The potential for decellularized aortic heart valves (AVs) as heart valve replacements is based on the assumption that the major cellular immunogenic components have been removed, and that the remaining extracellular matrix (ECM) should retain the necessary mechanical properties and functional design. However, decellularization processes likely alter the ECM mechanical and structural properties, potentially affecting long-term durability. In the present study, we explored the effects of an anionic detergent (sodium dodecyl sulfate (SDS)), enzymatic agent (Trypsin), and a non-ionic detergent (Triton X-100) on the mechanical and structural properties of AV leaflets (AVLs) to provide greater insight into the initial functional state of the decellularized AVL. The overall extensibility represented by the areal strain under 60 N/m increased from 68.85% for the native AV to 139.95%, 137.51%, and 177.69% for SDS, Trypsin, and Triton X-100, respectively, after decellularization. In flexure, decellularized AVLs demonstrated a profound loss of stiffness overall, and also produced a nonlinear moment–curvature relation compared to the linear response of the native AVL. Effective flexural moduli decreased from 156.0±24.6 kPa for the native AV to 23.5±5.8, 15.6±4.8, and 19.4±8.9 kPa for SDS, Trypsin, and Triton X-100 treated leaflets, respectively. While the overall leaflet fiber architecture remained relatively unchanged, decellularization resulted in substantial microscopic disruption. In conclusion, changes in mechanical and structural properties of decellularized leaflets were likely associated with disruption of the ECM, which may impact the durability of the leaflets.

Keywords
Heart valves; Tissue engineering; Decellularization; Collagen fibers; Biomechanics; Aortic valve
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Effects of decellularization on the mechanical and structural properties of the porcine aortic valve leaflet
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 8, March 2008, Pages 1065–1074
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us