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The development and characterization of a glutathione-sensitive cross-linked polyethylenimine gene vector

Paper ID Volume ID Publish Year Pages File Format Full-Text
11301 730 2006 7 PDF Available
Title
The development and characterization of a glutathione-sensitive cross-linked polyethylenimine gene vector
Abstract

A glutathione-sensitive cross-linked polyethylenimine gene vector CLPEI50% was specially designed via the cross-linking reaction between the low molecular weight polyethylenimine (PEI1800) and dimethyl 3.3′-dithiopropionimidate dihydrochloride (DTBP). The acid–base titration test indicated that CLPEI50% still possessed efficient proton sponge effect. The property of CLPEI50%–DNA complexes were investigated by atomic force microscopy (AFM) and dynamic light scattering (DLS). CLPEI50% induced DNA condensation and formed spherical nanoparticles. The diameter of polyplexes prepared at pH value of 6.0 and 7.4 was about 150 and 260 nm, respectively. It was interesting to find the polyplexes were sensitive to the reductive glutathione (GSH). The CLPEI50%–DNA polyplexes prepared at N/P ratio of 10 were unpacked at GSH concentration of 3 mm, which was comparable to the intracellular environment. The in vitro cytotoxicity of CLPEI50% was also significantly reduced comparing with PEI25k. The biomimetic CLPEI50%–DNA polyplexes with the low cytotoxicity and GSH-sensitive property could be a good candidate for gene delivery.

Keywords
Gene delivery; Biomimetic; Glutathione-sensitive; Low cytotoxicity
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The development and characterization of a glutathione-sensitive cross-linked polyethylenimine gene vector
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 30, October 2006, Pages 5292–5298
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us