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Electrical coupling of cardiomyocyte sheets occurs rapidly via functional gap junction formation

Paper ID Volume ID Publish Year Pages File Format Full-Text
11318 731 2006 10 PDF Available
Title
Electrical coupling of cardiomyocyte sheets occurs rapidly via functional gap junction formation
Abstract

Previously, we have successfully created pulsatile myocardial tissue grafts using our novel technology, “cell sheet engineering”, that layers cell sheets fabricated on temperature-responsive culture dishes to form three-dimensional (3-D) structures. Electrical coupling is established between layered neonatal rat cardiomyocyte sheets, resulting in the synchronized beating of 3-D myocardial tissues. However, the mechanism by which these layered cardiomyocyte sheets communicate electrically is not well-understood. In this study, we used a multiple-electrode extracellular recording system and demonstrated that bilayer cardiomyocyte sheets coupled electrically with slight delays 34±2 min (mean±SEM) after layering. These delays gradually decreased and the electrical actions of layered cell sheets were completely coupled 46±3 min (mean±SEM) after initial layering. Immunohistological analysis showed that connexin43, a gap junction (GJ)-related protein, existed not only at cell-to-cell interfaces but also on the free cell membrane in the cardiomyocyte sheet. Additionally, neither connexin40 nor connexin45, but only connexin43 was detected between bilayer cardiomyocyte sheets within 30 min after layering. Dye transfer assay demonstrated that the exchange of small molecules via GJs occurred within 30 min. The cell sheet manipulation technique using the temperature-responsive culture dishes has substantial advances and the exciting potential in the fields of cell and tissue physiology, as well as tissue engineering.

Keywords
Cardiac tissue engineering; Electrophysiology; Immunochemistry
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 27, September 2006, Pages 4765–4774
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us