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Enhanced selective immobilization of biomolecules onto solid supports coated with semifluorinated self-assembled monolayers

Paper ID Volume ID Publish Year Pages File Format Full-Text
11349 734 2008 11 PDF Available
Title
Enhanced selective immobilization of biomolecules onto solid supports coated with semifluorinated self-assembled monolayers
Abstract

A new strategy for the specific immobilization of proteins onto a solid support is presented. The immobilization is highly specific and efficient thanks to functionalized semifluorinated self-assembled monolayers (SAMs), thus reducing non-specific protein adsorption. Protein resistance does not exclusively result from classical steric repulsions and the presence of the both hydrophobic and lipophobic rigid and helical fluorinated cylinder in the depth of the SAMs does play an active role in protein repulsion. That repulsive effect is of higher magnitude than that of polyethylene glycol (PEG) as capping of the semifluorinated SAMs with additional ethylene oxide units provokes an attenuation of protein resistance. Though specific binding capacity of SAMs is intrinsically limited, the fluorinated SAMs described allow sensitive measurements with lower limits of detection and higher signal-to-noise ratios when compared to the best chips so far used in surface plasmon resonance (SPR) analysis.

Keywords
Fluorocarbon; Self-assembly; Surface modification; Protein adsorption; Plasma protein; Biosensor
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Enhanced selective immobilization of biomolecules onto solid supports coated with semifluorinated self-assembled monolayers
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 29, Issue 2, January 2008, Pages 204–214
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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