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A study of thermoresponsive poly(N-isopropylacrylamide)/polyarginine bioconjugate non-viral transgene vectors

Paper ID Volume ID Publish Year Pages File Format Full-Text
11372 735 2006 9 PDF Available
Title
A study of thermoresponsive poly(N-isopropylacrylamide)/polyarginine bioconjugate non-viral transgene vectors
Abstract

A thermoresponsive poly(N-isopropylacrylamide)/poly(l-arginine)bioconjugate (PNIPArg) was prepared by radical polymerization and EDC-activated coupling. The lower critical solution temperature (LCST) of PNIPArg aqueous solution determined by turbidimetry was around 35.2 °C. The transmission electron microscope (TEM) showed that the PNIPArg/DNA complexes appeared uniform nanospheres with size about 50–120 nm. Variable temperature circular dichroism (CD) and gel electrophoresis results revealed that the association and dissociation of PNIPArg/DNA complexes could be tuned by varying temperature; polyarginine (PArg) showed no temperature-controllable change of DNA condensate. Incorporation of PNIPAAm considerably decreased the cytotoxicity of PArg. The transfection level of PNIPArg and PArg was evaluated with COS-1 cells using two different reporter genes, pGL3-Control encoding luciferase and pEGP-C1 encoding green fluorescent protein (GFP). The transfection efficiency of PNIPArg incubated at 37 °C for 22 h, 20 °C for 2 h and 37 °C for 24 h was enhanced to a different extent depending on PNIPArg/DNA ratios compared to that incubated at 37 °C for 48 h. Encouragingly, at PNIPArg/DNA mass ratio of 3/1, the transfection efficiency of PNIPArg obtained with variable temperature route was equivalent to that of LipofectamineTM 2000.

Keywords
Poly(N-isopropylacrylamide); Poly(l-arginine); Thermoresponsive; Non-viral vector; Gene transfection
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A study of thermoresponsive poly(N-isopropylacrylamide)/polyarginine bioconjugate non-viral transgene vectors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 28, October 2006, Pages 4984–4992
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us