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Directed growth and selective differentiation of neural progenitor cells on micropatterned polymer substrates

Paper ID Volume ID Publish Year Pages File Format Full-Text
11380 736 2006 11 PDF Available
Title
Directed growth and selective differentiation of neural progenitor cells on micropatterned polymer substrates
Abstract

Directional growth and differentiation of adult rat hippocampal progenitor cells (AHPCs) were investigated on micropatterned polymer substrates in vitro. Astrocytes or AHPCs cultured on micropatterned polystyrene substrates chemically modified with laminin exhibited over 75% alignment in the groove direction. AHPCs co-cultured with astrocytes preferentially acquired neuronal morphology, with nearly double the percentage of cells expressing class III β-tubulin on the micropatterned half of the substrate, as opposed to the planar half of the substrate, or compared to those growing in the absence of astrocytes. This indicates that substrate three-dimensional topography, in synergy with chemical (laminin) and biological (astrocytes) guidance cues, facilitates neuronal differentiation of the AHPCs. Through multi-dimensional cell–cell interactions, this environment provides spatial control selectively enhancing neuronal differentiation and neurite alignment on topographically different regions of the same substrate. Integrating these cues is important in understanding and controlling neural stem cell differentiation and designing scaffolds for guided nerve regeneration.

Keywords
Neural stem/progenitor cell; Astrocyte; Co-culture; Micropatterning; Nerve tissue engineering
First Page Preview
Directed growth and selective differentiation of neural progenitor cells on micropatterned polymer substrates
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 22, August 2006, Pages 4098–4108
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering