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Detection of a specific DNA sequence by electrophoresis through a molecularly imprinted polymer

Paper ID Volume ID Publish Year Pages File Format Full-Text
11389 736 2006 6 PDF Available
Title
Detection of a specific DNA sequence by electrophoresis through a molecularly imprinted polymer
Abstract

To develop a simple and inexpensive DNA detection method, we prepared a molecularly imprinted polymer (MIP) gel for recognizing a specific double-stranded DNA (dsDNA) target sequence in MIP gel electrophoresis (MIPGE). During MIPGE, migration of the target sequence of dsDNA should be hindered by the capture effect of the binding sites in the MIP gel. This migration hindrance of target dsDNA was determined by plotting the relationship between the migration distance in the MIP gel and that in polyacrylamide gel, commonly used in gel electrophoresis. Using this plot, detection of a target dsDNA from a mixture of different-sized dsDNA fragments was achieved. Moreover, we found the detection method successfully distinguished between a target and its base-pair substitutes. These results suggest that MIPGE could be employed for detection of a target dsDNA sequence.

Keywords
Biomimetic material; Biosensor; DNA; Hydrogel; Polymerization
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Detection of a specific DNA sequence by electrophoresis through a molecularly imprinted polymer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 22, August 2006, Pages 4177–4182
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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