fulltext.study @t Gmail

Osteoblast functions on functionalized PMMA-based polymers exhibiting Staphylococcus aureus adhesion inhibition

Paper ID Volume ID Publish Year Pages File Format Full-Text
11397 737 2006 8 PDF Available
Title
Osteoblast functions on functionalized PMMA-based polymers exhibiting Staphylococcus aureus adhesion inhibition
Abstract

Staphylococcus aureus adhesion and osteoblast functions were assessed on functionalized poly(methyl methacrylate)-based terpolymers bearing randomly distributed carboxylate and sulfonate groups. These terpolymers were synthesized by radical polymerization, characterized by nuclear resonance spectroscopy and classified by the ratio R=[COO−/COO−+SO3−] in the range 0.5–0.8. Bacterial adhesion study showed that fibronectin-coated terpolymers with R varying from 0.5 to 0.8 exhibited inhibition rate of S. aureus adhesion from 90% to 98% as compared to the adhesion on unfunctionalized poly(methyl methacrylate). In contrast, the adhesion of osteoblasts onto the same functionalized terpolymers was decreased by 20% when compared to the results obtained on poly(methyl methacrylate). While the amount of attached osteoblasts are similar onto all the functionalized terpolymers whatever its R value, the cell proliferation was different and was found to vary with R in the range 0.5–0.8. Osteoblast proliferation, alkaline phosphatase activity and accumulation of calcium in the extracellular matrix of these cells, cultured on the functionalized terpolymers with R equal to 0.7–0.8 were similar to that observed onto non-functionalized poly(methyl methacrylate). In contrast, osteoblast proliferation was inhibited on terpolymers with an R value around 0.6. These results provide evidence that functionalized poly(methyl methacrylate)-based terpolymers with R ratio equaling 0.7–0.8 simultaneously inhibit bacteria adhesion and support osteoblast functions pertinent to new bone formation. These functionalized polymers could, therefore, be used as coating or grafted on orthopedic and dental implants to render them both bone compatible and able to prevent bacterial infection.

Keywords
Functionalized PMMA-based polymers; Osteoblast proliferation; Osteoblast phenotype; Bacterial adhesion; S. aureus
First Page Preview
Osteoblast functions on functionalized PMMA-based polymers exhibiting Staphylococcus aureus adhesion inhibition
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 21, July 2006, Pages 3912–3919
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us