fulltext.study @t Gmail

Electrospinning of chitin nanofibers: Degradation behavior and cellular response to normal human keratinocytes and fibroblasts

Paper ID Volume ID Publish Year Pages File Format Full-Text
11400 737 2006 11 PDF Available
Title
Electrospinning of chitin nanofibers: Degradation behavior and cellular response to normal human keratinocytes and fibroblasts
Abstract

An electrospinning method was used to fabricate chitin nanofibrous matrices for biodegradability and cell behavior tests. The morphology of as-spun chitin nanofibers (Chi-N) and commercial chitin microfibers (Beschitin W®; Chi-M) was investigated by scanning electron microscopy. From the image analysis, the average diameters of Chi-N and Chi-M were 163 nm and 8.77 μm, respectively. During in vitro degradation for 15 days, the degradation rate of Chi-N was faster than that of Chi-M, likely due to higher surface area of Chi-N. Chi-N that was grafted into rat subcutaneous tissue had almost degraded within 28 days, and no inflammation could be seen on the nanofiber surfaces or in the surrounding tissues (except in the early stage wound). To assay and compare the cytocompatibility and cell behavior with Chi-N and Chi-M, cell attachment and spreading of normal human keratinocytes and fibroblasts seeded on chitin matrices and the interaction between cells and chitin fibers were studied. Relatively high cell attachment and spreading of all the cells tested were observed on Chi-N in comparison to Chi-M, and Chi-N treated with type I collagen significantly promoted the cellular response. Our results indicate that the Chi-N, alone or with extracellular matrix proteins (particularly type I collagen), could be potential candidates for the cell attachment and spreading of normal human keratinocytes and fibroblasts. This property of Chi-N might be particularly useful for wound healing and regeneration of oral mucosa and skin.

Keywords
Electrospinning; Chitin nanofibers; Degradation; Extracellular matrix protein; Cell behavior
First Page Preview
Electrospinning of chitin nanofibers: Degradation behavior and cellular response to normal human keratinocytes and fibroblasts
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 21, July 2006, Pages 3934–3944
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering