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Surface immobilization of antibody on silk fibroin through conformational transition

Paper ID Volume ID Publish Year Pages File Format Full-Text
1142 77 2011 5 PDF Available
Title
Surface immobilization of antibody on silk fibroin through conformational transition
Abstract

In recent studies silk fibroin has been explored as a new material platform for biosensors. Based on these developments, a procedure for the immobilization of antibodies on silk fibroin substrates was developed as a route to functionalizing these biosensor systems. By controlling the conformational transition of the silk fibroin, a primary antibody was immobilized and enriched at the surface of silk fibroin substrates under mild reaction conditions to maintain antibody function. Compared to chemical crosslinking, the immobilization efficiency in the present approach was increased significantly. This method, achieving high loading of antibody while retaining function, improves the feasibility of silk fibroin as a platform material for biosensor applications.

Keywords
Silk fibroin; Antibody; Biosensors; Immobilization; Stability
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Surface immobilization of antibody on silk fibroin through conformational transition
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 7, July 2011, Pages 2782–2786
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us