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Modulation of collagen II fiber formation in 3-D porous scaffold environments

Paper ID Volume ID Publish Year Pages File Format Full-Text
1145 77 2011 13 PDF Available
Title
Modulation of collagen II fiber formation in 3-D porous scaffold environments
Abstract

Collagen II, a major extracellular matrix component in cartilaginous tissues, undergoes fibrillogenesis under physiological conditions. The present study explored collagen II fiber formation in solution and in two- (coverslip) and three-dimensional (scaffold) environments under different incubation conditions. These conditions include variations in adsorption buffers, the presence of 1-ethyl-3-(3-dimenthylaminopropyl) carbodiimide/N-hydroxysuccinimide crosslinker and the nature of the material surfaces. We extend our observations of collagen II fiber formation in two dimensions to develop an approach for the formation of a fibrillar collagen II network throughout surface-modified polylactide-co-glycolide porous scaffolds. Morphologically, the collagen II network is similar to that present in native articular cartilage. Biological validation of the resultant optimized functional scaffold, using rat bone marrow-derived mesenchymal stem cells, shows appreciable cell infiltration throughout the scaffold with enhanced cell spreading at 24 h post-seeding. This economic and versatile approach is thus believed to have significant potential in cartilage tissue engineering applications.

Keywords
Collagen II; Fibrillogenesis; EDAC/NHS crosslinking; 2-D and 3-D environments; Surface modification
First Page Preview
Modulation of collagen II fiber formation in 3-D porous scaffold environments
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 7, July 2011, Pages 2804–2816
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering