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Engineered matrix coatings to modulate the adhesion of CD133+ human hematopoietic progenitor cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
11464 742 2007 8 PDF Available
Title
Engineered matrix coatings to modulate the adhesion of CD133+ human hematopoietic progenitor cells
Abstract

Interactions of hematopoietic progenitor cells (HPC) with their local microenvironments in the bone marrow are thought to control homing, differentiation, and self-renewal of the cells. To dissect the role of extracellular matrix (ECM) components of the niche microenvironment, a set of well-defined ECM coatings including fibronectin, heparin, heparan sulphate, hyaluronic acid, tropocollagen I, and co-fibrils of collagen I with heparin or hyaluronic acid was prepared and analysed with respect to the attachment of human CD133+ HPC in vitro. The extension of the adhesion areas of individual cells as well as the fraction of adherent cells were assessed by reflection interference contrast microscopy (RICM). Intense cell–matrix interactions were found on surfaces coated with fibronectin, heparin, heparan sulphate, and on the collagen I based co-fibrils. Insignificant adhesion was found for tropocollagen I and hyaluronic acid. The strongest adhesion of HPC was observed on fibronectin with contact areas of about 7 μm2. Interaction of HPC with coatings consisting of heparin, heparan sulphate, and co-fibrils result in small circular shaped contact zones of 3 μm2 pointing to another, less efficient, adhesion mechanism. Analysing the specificity of cell–matrix interaction by antibody blocking experiments suggests an integrin(α5β1)-specific adhesion on fibronectin, while adhesion on heparin was shown to be mediated by selectins (CD62L). Taken together, our data provide a basis for the design of advanced culture carriers supporting site-specific proliferation or differentiation of HPC.

Keywords
Hematopoietic stem cell; Cell adhesion; Fibronectin; Heparin; Hyaluronic acid; Collagen
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Engineered matrix coatings to modulate the adhesion of CD133+ human hematopoietic progenitor cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 5, February 2007, Pages 836–843
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us