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In vitro biodegradability and mechanical properties of bioabsorbable bacterial cellulose incorporating cellulases

Paper ID Volume ID Publish Year Pages File Format Full-Text
1148 77 2011 11 PDF Available
Title
In vitro biodegradability and mechanical properties of bioabsorbable bacterial cellulose incorporating cellulases
Abstract

Bacterially produced cellulose is being actively studied as a novel scaffold material for wound care and tissue engineering applications. Bioabsorbability of the scaffold material is desired to enable improved restoration of targeted tissue. Recently, a bioabsorbable bacterial cellulose (BBC) incorporating cellulase enzymes has been demonstrated. It was revealed that some cellulases may lose up to 90% of their activity if present in a suboptimal pH environment. Therefore, a key challenge in the practical implementation of this approach rests in compensating for the variation in the wound or tissue pH, which may significantly reduce the activity of some enzymes. In this work, buffer ingredients were incorporated into the bacterial cellulose in order to create a more optimal pH microenvironment for the preferred acid cellulases, which are significantly less active at the biological pH 7.4. The results demonstrated that incorporation of buffer ingredients helped to retain the activity of the cellulases. The glucose released from degraded materials was also increased from 30% without incorporation of buffer ingredients to 97% in the presence of incorporated buffer ingredients at the suboptimal pH environment of 7.4. The use of simulated body fluid and simulated tissue padding, both mimicking the real wound environment, also demonstrated some improvements in terms of material degradation. Measurements of mechanical properties of materials revealed that BBC materials have tensile strength and extensibility similar to human skin, especially when hydrated with saline water prior to use.

Keywords
Bacterial cellulose; Cellulase; Bioabsorbability; Wound care; Tissue scaffold
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In vitro biodegradability and mechanical properties of bioabsorbable bacterial cellulose incorporating cellulases
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 7, July 2011, Pages 2835–2845
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us