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Cell adhesion on a polymerized peptide–amphiphile monolayer

Paper ID Volume ID Publish Year Pages File Format Full-Text
11534 747 2006 11 PDF Available
Title
Cell adhesion on a polymerized peptide–amphiphile monolayer
Abstract

We report the synthesis and characterization of a stable polymerized monolayer of peptide–amphihiles on a planar solid support that promotes mouse fibroblast cell adhesion and spreading. Peptide–amphiphiles consisting of a polymerizable fatty acid attached to a short RGD containing peptide sequence are self-assembled and polymerized at the water–air interface by means of the Langmuir– Blodgett technique. The surface concentration of the peptide–amphiphile is varied by co-spreading the peptide–amphiphile with an analogous non-modified polymerizable amphiphile at the water/air interface, prior to UV light-induced polymerization. The polymerized monolayer is transferred onto a hydrophobized smooth mica surface and the resulting surfaces have been investigated with respect to directing the cell adhesion and spreading of mouse fibroblast cells in a serum-free medium. Fibroblast cells adhere and spread on surfaces exposing the bioactive ligand but do not spread on reference surfaces without peptide. We find a maximum number of adherent cells at rather high peptide surface concentrations of about 10 mol% in the mixed monolayer, equivalent to more than 50 pmol/cm2 peptide on the surface of the film. We attribute this finding to a limited accessibility of the ligands by the integrins. Because of the stability of the polymerized peptide–amphiphile monolayer, these surfaces can be re-seeded multiple times with cells, i.e. adherent cells can be removed from the surface, the surface can be sterilized and cells can be re-attached.

Keywords
RGD peptide; Cell adhesion; Polymerization; Langmuir–Blodgett film
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Cell adhesion on a polymerized peptide–amphiphile monolayer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 8, March 2006, Pages 1259–1269
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us