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Micropatterned cell co-cultures using layer-by-layer deposition of extracellular matrix components

Paper ID Volume ID Publish Year Pages File Format Full-Text
11558 747 2006 8 PDF Available
Title
Micropatterned cell co-cultures using layer-by-layer deposition of extracellular matrix components
Abstract

Micropatterned cellular co-cultures were fabricated using three major extracellular matrix components: hyaluronic acid (HA), fibronectin (FN) and collagen. To fabricate co-cultures with these components, HA was micropatterned on a glass substrate by capillary force lithography, and the regions of exposed glass were coated with FN to generate cell adhesive islands. Once the first cell type was immobilized on the adhesive islands, the subsequent electrostatic adsorption of collagen to HA patterns switched the non-adherent HA surfaces to adherent, thereby facilitating the adhesion of a second cell type. This technique utilized native extracellular matrix components and therefore affords high biological affinity and no cytotoxicity. This biocompatible co-culture system could potentially provide a new tool to study cell behavior such as cell–cell communication and cell–matrix interactions, as well as tissue-engineering applications.

Keywords
Patterned co-culture; Layer-by-layer; Hyaluronic acid; Fibronectin; Collagen
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Micropatterned cell co-cultures using layer-by-layer deposition of extracellular matrix components
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 8, March 2006, Pages 1479–1486
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us