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Polystyrene replicas of neuronal basal lamina act as excellent guides for regenerating neurites

Paper ID Volume ID Publish Year Pages File Format Full-Text
1156 77 2011 9 PDF Available
Title
Polystyrene replicas of neuronal basal lamina act as excellent guides for regenerating neurites
Abstract

Various scaffolds, natural or artificial, have been used for neural repair, including basal lamina scaffolds obtained through extraction of nerves. Here we tested whether plastic casts of such preparations could be used for neurite guidance. To this end, longitudinal micron thick sections of rat sciatic nerve were extracted with detergents and treated with Dnase, yielding an acellular basal lamina master. From the basal lamina master a polydimethylsiloxane (PDMS) mold was made. Then a polystyrene replica was made using the PDMS mold as the master. The polystyrene replica showed high similarity to the master within nanometer resolution as revealed by scanning electron microscopy. Organ cultured mouse dorsal root ganglia grown on the polystyrene replica and the master preparation exhibited guided outgrowth of neurites as assayed by two-dimensional fast Fourier transform analysis on preparations, where the neurites had been visualized by β-III-tubulin staining. The neurites aligned longitudally in the direction of the original basal lamina tubes. Thus, using inexpensive methods it is possible to make replicas of basal lamina which can be used for neurite guidance. This opens a new avenue for nerve reconstruction.

Keywords
Axon guidance; Basal lamina; Dorsal root ganglion; Extraction of nerves; Polystyrene
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Polystyrene replicas of neuronal basal lamina act as excellent guides for regenerating neurites
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 7, July 2011, Pages 2910–2918
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us