fulltext.study @t Gmail

Determination of human lens capsule permeability and its feasibility as a replacement for Bruch's membrane

Paper ID Volume ID Publish Year Pages File Format Full-Text
11580 747 2006 9 PDF Available
Title
Determination of human lens capsule permeability and its feasibility as a replacement for Bruch's membrane
Abstract

We have investigated human anterior lens capsule as a potential replacement for Bruch's membrane as a treatment for age-related macular degeneration. Any substrate to replace Bruch's membrane should possess certain characteristics to maintain proper function of the overlying retina. One of the important properties of Bruch's membrane is allowing the flow of nutrients and waste between the retinal pigment epithelium and the choriocapillaris. Here, we measured the permeability of the lens capsule by studying the diffusion of various molecular weight FITC–dextran molecules. Expressions for extraction of diffusion coefficients from concentration vs. time data from a blind-well chamber apparatus were derived for both a single and double membrane experiments. The diffusion coefficients in the lens capsule were found to be in the range of 10−6 to 10−10 cm2/s. We demonstrated a power law relationship, with the diffusion coefficient possessing a −0.6 order dependence on molecular weight. The molecular weight exclusion limit was determined to be 150±40 kDa. We have compared this value with reported values of Bruch's membrane molecular weight exclusion limit and find that the lens capsule has the potential to act as a substitute Bruch's membrane.

Keywords
Diffusion coefficient; Retina; Lens capsule; Dextran; Membrane
First Page Preview
Determination of human lens capsule permeability and its feasibility as a replacement for Bruch's membrane
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 8, March 2006, Pages 1670–1678
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering