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Sintered hydroxyfluorapatites—IV: the effect of fluoride substitutions upon colonisation of hydroxyapatites by mouse embryonic stem cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
11595 748 2004 10 PDF Available
Title
Sintered hydroxyfluorapatites—IV: the effect of fluoride substitutions upon colonisation of hydroxyapatites by mouse embryonic stem cells
Abstract

Biodegradable scaffolds serve a central role for tissue engineering scaffolds and guiding tissue regeneration. Some of these scaffolds, including apatites, display a significant effect upon cell adhesion and cell proliferation. The incorporation of scaffold technology with the developing embryonic stem (ES) cell field and the capacity of ES cells for self-renewal and differentiation are believed to hold enormous potential for applications in biomedical research and regenerative medicine.The purpose of this work was to determine the effect of hydroxyapatite (HAP) and fluoride substitutions of HAP upon ES cell growth and colonisation. Sintered hydroxyfluorapatite discs were found to support cellular proliferation and colonisation, and the ES cells displayed a tendency for differentiation on the apatite surface as determined by reductions in colony Oct4 immunoreactivity. Fluoride-containing HAPs were found to provide equivalent support to gelatin in terms of cell numbers, yet superior support for cellular colonisation when compared to HAP. This study indicates that fluoride substitutions of HAP may represent a viable strategy for the development of certain engineered tissue replacements and tissue regeneration systems using ES cells.

Keywords
Mouse embryonic stem (ES) cells; Proliferation; Colonisation; Differentiation; Hydroxyapatite; Fluoride; Tissue engineering scaffold
First Page Preview
Sintered hydroxyfluorapatites—IV: the effect of fluoride substitutions upon colonisation of hydroxyapatites by mouse embryonic stem cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 20, September 2004, Pages 4977–4986
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering