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Successful chondrogenesis within scaffolds, using magnetic stem cell confinement and bioreactor maturation

Paper ID Volume ID Publish Year Pages File Format Full-Text
116 10 2016 10 PDF Available
Title
Successful chondrogenesis within scaffolds, using magnetic stem cell confinement and bioreactor maturation
Abstract

Tissue engineering strategies, such as cellularized scaffolds approaches, have been explored for cartilage replacement. The challenge, however, remains to produce a cartilaginous tissue incorporating functional chondrocytes and being large and thick enough to be compatible with the replacement of articular defects. Here, we achieved unprecedented cartilage tissue production into a porous polysaccharide scaffold by combining of efficient magnetic condensation of mesenchymal stem cells, and dynamic maturation in a bioreactor. In optimal conditions, all the hallmarks of chondrogenesis were enhanced with a 50-fold increase in collagen II expression compared to negative control, an overexpression of aggrecan and collagen XI, and a very low expression of collagen I and RUNX2. Histological staining showed a large number of cellular aggregates, as well as an increased proteoglycan synthesis by chondrocytes. Interestingly, electron microscopy showed larger chondrocytes and a more abundant extracellular matrix. In addition, the periodicity of the neosynthesized collagen fibers matched that of collagen II. These results represent a major step forward in replacement tissue for cartilage defects.Statement of SignificanceA combination of several innovative technologies (magnetic cell seeding, polysaccharide porous scaffolds, and dynamic maturation in bioreactor) enabled unprecedented successful chondrogenesis within scaffolds.

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Keywords
Magnetic mesenchymal stem cells; Chondrogenesis; Cartilage defect; Bioreactor; Tissue engineering
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Successful chondrogenesis within scaffolds, using magnetic stem cell confinement and bioreactor maturation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 37, June 2016, Pages 101–110
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us