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Three-dimensional (3-D) imaging of chondrocytes in articular cartilage: Growth-associated changes in cell organization

Paper ID Volume ID Publish Year Pages File Format Full-Text
11639 750 2007 10 PDF Available
Title
Three-dimensional (3-D) imaging of chondrocytes in articular cartilage: Growth-associated changes in cell organization
Abstract

Three-dimensional (3-D) imaging and analysis techniques can be used to assess the organization of cells in biological tissues, providing key insights into the role of cell arrangement in growth, homeostasis, and degeneration. The objective of the present study was to use such methods to assess the growth-related changes in cell organization of articular cartilage from different sites in the bovine knee. Three-dimensional images of fetal, calf, and adult cartilage were obtained and processed to identify cell nuclei. The density of cells was lower with growth and with increasing depth from the articular surface. The cell organization, assessed by the angle to the nearest neighboring cell, also varied with growth, and reflected the classical organization of cells in adult tissue, with neighboring cells arranged horizontally in the superficial zone (average angle of 20°) and vertically in the deep zone (60°). In all other regions and growth stages of cartilage, the angle was ∼32°, indicative of an isotropic organization. On the contrary, the nearest neighbor distance did not vary significantly with growth or depth. Together, these results indicate that cartilage growth is associated with distinctive 3-D arrangements of groups of chondrocytes.

Keywords
Cartilage; Cell organization; Chondrocyte; Growth; Image analysis
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Three-dimensional (3-D) imaging of chondrocytes in articular cartilage: Growth-associated changes in cell organization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 28, Issue 2, January 2007, Pages 230–239
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us