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Bone tissue engineering evaluation based on rat calvaria stromal cells cultured on modified PLGA scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
11661 751 2006 9 PDF Available
Title
Bone tissue engineering evaluation based on rat calvaria stromal cells cultured on modified PLGA scaffolds
Abstract

Using natural materials to coat the scaffolds used for tissue-engineered bone-repair techniques is expected to increase osteoblast adhesion to the scaffold and to express normal physiological function. To test this hypothesis, we therefore modified poly(dl-lactic-co-glycolic acid) (PLGA) substrate by coating it with natural biomaterial solutions of collagen, chitosan, or N-succinyl-chitosan, and then used these three combinations as scaffolds to evaluate their effects on osteoblast attachment, proliferation, and differentiation. The results demonstrated that the pore size of scaffolds ranging from 125–500 μm did not affect the osteoblast phenotype; however, the surface modification of the scaffolds coated with these natural biomaterials did. Collagen increased cell attachment and proliferation, but chitosan and N-succinyl-chitosan decreased them. Chitosan and N-succinyl-chitosan increased differentiation, but collagen decreased it. These results provide us a new strategy for modifying microenvironments to increase osteoblast adhesion, proliferation, and differentiation on PLGA scaffolds, a strategy that might be useful for tissue regeneration.

Keywords
Bone tissue engineering; Poly(lactic-co-glycolic acid); Chitosan; N-succinyl-chitosan; Collagen; Scaffold
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Bone tissue engineering evaluation based on rat calvaria stromal cells cultured on modified PLGA scaffolds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 6, February 2006, Pages 896–904
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us