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Effective transfection of cells with multi-shell calcium phosphate-DNA nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
11691 753 2006 7 PDF Available
Title
Effective transfection of cells with multi-shell calcium phosphate-DNA nanoparticles
Abstract

Coated calcium phosphate nanoparticles were prepared for cell transfection. A calcium phosphate nanoparticle served as core which was then coated with DNA for colloidal stabilisation. The efficiency of transfection could be considerably increased by adding another layer of calcium phosphate on the surface, thereby incorporating DNA into the particle and preventing its degradation within the cell by lysosomes. A subsequent outermost layer of DNA on the calcium phosphate gave a colloidal stabilisation. The efficiency of such multi-shell particles was significantly higher than that of simple DNA-coated calcium phosphate nanoparticles. The transfection efficiency of EGFP-encoding DNA was tested with different cell lines (T-HUVEC, HeLa, and LTK). The dispersions were stable and could be used for transfection after 2 weeks of storage at 4 °C without loss of efficiency.

Keywords
Calcium phosphate; DNA; Transfection; Drug delivery; Gene therapy
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Effective transfection of cells with multi-shell calcium phosphate-DNA nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 16, June 2006, Pages 3147–3153
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us