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Time-dependent morphology and adhesion of osteoblastic cells on titanium model surfaces featuring scale-resolved topography

Paper ID Volume ID Publish Year Pages File Format Full-Text
11694 754 2004 17 PDF Available
Title
Time-dependent morphology and adhesion of osteoblastic cells on titanium model surfaces featuring scale-resolved topography
Abstract

The role of micrometer and submicrometer surface roughness on the interaction of cells with titanium model surfaces of well-defined topography was investigated using human bone-derived cells (MG63 cells). The early phase of interactions was studied using a kinetic morphological analysis of adhesion, spreading and proliferation of the cells. By SEM and double immunofluorescent labeling of vinculin and actin, it was found that the cells responded to nanoscale roughness by a higher cell thickness and a delayed apparition of the focal contacts. A singular behavior was observed on nanoporous oxide surfaces, where the cells were more spread and displayed longer and more numerous filopods. On electrochemically microstructured surfaces with hemispherical cavities, arranged in a hexagonal pattern, the MG63 cells were able to go inside, adhere and proliferate in cavities of 30 or 100 μm in diameter, whereas they did not recognize the 10 μm diameter cavities. Cells adopted a 3D shape when attaching inside the 30 μm diameter cavities. Condensation of actin cytoskeleton correlated with vinculin-positive focal contacts on cavity edges were observed on all microstructured surfaces. Nanotopography on surfaces with 30 μm diameter cavities had little effect on cell morphology compared to flat surfaces with same nanostructure, but cell proliferation exhibited a marked synergistic effect of microscale and nanoscale topography.

Keywords
Osteoblast; Cell adhesion; Cell morphology; Cell proliferation; Surface topography; Titanium
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Time-dependent morphology and adhesion of osteoblastic cells on titanium model surfaces featuring scale-resolved topography
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 14, June 2004, Pages 2695–2711
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us