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Promotion of fibroblast activity by coating with hydrophobins in the β-sheet end state

Paper ID Volume ID Publish Year Pages File Format Full-Text
11697 754 2004 9 PDF Available
Title
Promotion of fibroblast activity by coating with hydrophobins in the β-sheet end state
Abstract

Hydrophobins such as SC3 and SC4 of Schizophyllum commune self-assemble into an amphipathic film at hydrophilic/hydrophobic interfaces. These proteins can thus change the nature of surfaces, which makes them attractive candidates to improve physio– and physico–chemical properties of implant surfaces. At a hydrophobic solid, assembly of the hydrophobin is arrested in an intermediate state, called the α-helical state. The conversion to the stable β-sheet end state can be induced by treating the solid at elevated temperatures in the presence of detergent. We here show that SC3 and SC4 in the α-helical state homogeneously cover Teflon sheets when coating was performed at 20°C. However, when the protein was adsorbed at 80°C aggregates were shown to bind tightly to the adsorbed hydrophobin film. The transition to the β-sheet state created pores of about 50 nm in the SC3 and SC4 coatings when coating was performed at 20°C. Cell growth and morphology on SC4 coatings was better than on SC3. In case of both hydrophobins, fibroblast growth and morphology was not influenced by the coating temperature or the conformation of the protein. However, in contrast to the α-helical state, the β-sheet state of both SC3 and SC4 hardly, if at all, affected mitochondrial activity.

Keywords
Hydrophobin; Protein coating; Biocompatibility; Fibroblast growth; Surface modification
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Promotion of fibroblast activity by coating with hydrophobins in the β-sheet end state
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 14, June 2004, Pages 2731–2739
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us