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Fluid shear in viscous fibronectin gels allows aggregation of fibrous materials for CNS tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
11700 754 2004 11 PDF Available
Title
Fluid shear in viscous fibronectin gels allows aggregation of fibrous materials for CNS tissue engineering
Abstract

Fibronectin (Fn) materials prepared from human plasma have been used in various forms as substrates for tissue engineering. Such purposes require that the soluble protein aggregates into insoluble fibrous structures which encourage the attachment and migration of cells. The method of aggregation due to mechanical shear was investigated by applying fluid shear forces directly to a viscous solution of Fn. Structural analysis revealed that mechanical shear resulted in the formation of an orientated fibrous protein material that was less soluble than its non-sheared counterpart. The suitability of this shear aggregated Fn material for CNS repair purposes was assessed in vitro where it supported the growth of fibroblasts, S100 immunoreactive Schwann cells and GFAP immunoreactive astrocytes. Implantation of the shear aggregated Fn material into a rat model of spinal cord injury provided a permissive environment for axonal growth. This was extended using an impermeable coating to improve orientation and straightness of axonal growth.

Keywords
Fibronectin; Protein aggregation; Spinal cord repair; Tissue engineering; Schwann cell; Neural fibroblast; Astrocyte
First Page Preview
Fluid shear in viscous fibronectin gels allows aggregation of fibrous materials for CNS tissue engineering
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 14, June 2004, Pages 2769–2779
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering