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Stimulation of in vivo angiogenesis by cytokine-loaded hyaluronic acid hydrogel implants

Paper ID Volume ID Publish Year Pages File Format Full-Text
11702 754 2004 10 PDF Available
Title
Stimulation of in vivo angiogenesis by cytokine-loaded hyaluronic acid hydrogel implants
Abstract

Crosslinked hyaluronic acid (HA) hydrogels were evaluated for their ability to elicit new microvessel growth in vivo when preloaded with one of two cytokines, vascular endothelial growth factor (VEGF) or basic fibroblast growth factor (bFGF). HA film samples were surgically implanted in the ear pinnas of mice, and the ears retrieved 7 or 14 days post implantation. Histologic analysis showed that all groups receiving an implant demonstrated significantly more microvessel density than control ears undergoing surgery but receiving no implant (p<0.01). Moreover, aqueous administration of either growth factor produced substantially more vessel growth than an HA implant with no cytokine. However, the most striking result obtained was a dramatic synergistic interaction between HA and VEGF. Presentation of VEGF in crosslinked HA generated vessel density of NI=6.7 at day 14, where NI is a neovascularization index defined below, more than twice the effect of the sum of HA alone (NI=1.8) plus VEGF alone (NI=1.3). This was twice the vessel density generated by co-addition of HA and bFGF (NI=3.4, p<0.001). New therapeutic approaches for numerous pathologies could be notably enhanced by the localized, synergistic angiogenic response produced by release of VEGF from crosslinked HA films.

Keywords
Hyaluronic acid; Hydrogel; Growth factors; Cytokine; VEGF; bFGF; Angiogenesis
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Stimulation of in vivo angiogenesis by cytokine-loaded hyaluronic acid hydrogel implants
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 14, June 2004, Pages 2789–2798
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us