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Osteoblast response to PLGA tissue engineering scaffolds with PEO modified surface chemistries and demonstration of patterned cell response

Paper ID Volume ID Publish Year Pages File Format Full-Text
11705 754 2004 12 PDF Available
Title
Osteoblast response to PLGA tissue engineering scaffolds with PEO modified surface chemistries and demonstration of patterned cell response
Abstract

Because tissues are characterized by a well-defined three-dimensional arrangement of cells, tissue engineering scaffolds that facilitate the organization and differentiation of new tissue will have improved performance in comparison to scaffolds that only provide surfaces for cell attachment and growth. We hypothesize that instructions for cells can be incorporated into tissue engineering scaffolds by patterning the scaffold's architecture and surface chemistry. Our goals for the presented work were to collect data about cell response to three-dimensional, porous scaffolds with uniformly modified surfaces chemistries, and to demonstrate patterning of cell response by patterning surface chemistry.Our system was osteoblast response to poly(l-lactide-co-glycolide) scaffolds modified with poly(ethylene oxide) (PEO). Scaffolds were fabricated using the Three-Dimensional Printing™ (3DP™) process which has control over scaffolds properties to a resolution of ∼100 μm in all three dimensions. At higher PEO concentrations, adhesion, growth rates, and migration of rat osteoblasts were reduced; alkaline phosphate activity was increased, and cells were less spread and had microvilli. Patterned regions of low and high cell adhesion were demonstrated on scaffolds fabricated with 1 mm thick stripes of PEO and non-PEO regions.

Keywords
Cell patterning; Poly(ethylene oxide); Poly(lactic-co-glycolic acid); Three-dimensional printing; Tissue engineering scaffolds
First Page Preview
Osteoblast response to PLGA tissue engineering scaffolds with PEO modified surface chemistries and demonstration of patterned cell response
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 14, June 2004, Pages 2819–2830
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering