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The effect of the amelogenin fraction of enamel matrix proteins on fibroblast-mediated collagen matrix reorganization ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
11731 756 2006 8 PDF Available
Title
The effect of the amelogenin fraction of enamel matrix proteins on fibroblast-mediated collagen matrix reorganization ☆
Abstract

Enamel matrix proteins (EMP), extracted from developing porcine teeth, promote not only periodontal regeneration but also cutaneous wound healing presumably via the amelogenin fraction. Because it is unclear whether the effect of EMP can be ascribed to amelogenins, we compared EMP with recombinant amelogenin in the relaxed dermal equivalent (DE) in vitro model for early wound contraction. EMP and recombinant porcine amelogenin (rP172) at 1 mg/ml were incorporated into DEs composed of human dermal fibroblasts and a type I collagen matrix. The area reduction, as a measure of contraction, as well as fibroblast numbers and TGF-β  1 levels, were quantified over 7 days in culture in the presence of 10% foetal bovine serum. Both EMP and recombinant amelogenin increased contraction (p<0.005p<0.005) and fibroblast numbers (p<0.005p<0.005) compared with controls (acetic acid vehicle and 1 mg/ml porcine serum albumin) and the positive control TGF-β1 added at 10 ng/ml. Increased contraction with EMP and recombinant amelogenin was most pronounced after the first day of incubation and was associated with elevated (p<0.005p<0.005) TGF-β1 levels in conditioned medium. In conclusion, the amelogenin component of EMP augmented fibroblast-driven collagen matrix remodelling, at least partially, by increasing the endogenous production of TGF-β1. These effects of EMP/amelogenin may be beneficial for cutaneous wound healing.

Keywords
Adhesion molecule; Amelogenin; Apoptosis; Extracellular matrix (ECM); MTT assay; TGF-β1
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The effect of the amelogenin fraction of enamel matrix proteins on fibroblast-mediated collagen matrix reorganization ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 15, May 2006, Pages 2926–2933
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us