fulltext.study @t Gmail

The modulation of gene expression in osteoblasts by thrombin coated on biphasic calcium phosphate ceramic

Paper ID Volume ID Publish Year Pages File Format Full-Text
11732 756 2006 10 PDF Available
Title
The modulation of gene expression in osteoblasts by thrombin coated on biphasic calcium phosphate ceramic
Abstract

For many years, fibrin sealants were associated with bone substitutes to promote bone healing. However, the osteoblastic response to fibrin sealant components remains poorly documented. In this study, MC3T3-E1 osteoblastic cells were cultured on biphasic calcium phosphate ceramic (MBCP®) coated with Tissucol® components (thrombin and fibrinogen). Analysis of osteoblastic differentiation markers by RT-PCR revealed that MBCP® coated with Tissucol® stimulated mRNA levels for osteocalcin and alkaline phosphatase (ALP). Of all the components of Tissucol®, thrombin has been reported to affect osteoblastic behavior. Our results demonstrated that low thrombin concentrations (0.5–5 U/ml) stimulated mRNA levels for ALP, whereas high thrombin concentrations (50–100 U/ml) decreased mRNA levels for ALP and PTH/PTHrP receptor and also increased mRNA level for the osteoclastogenesis inhibitor OPG. As thrombin stimulated angiogenesis, we then wondered whether thrombin could influence the expression of angiogenic factors. Low thrombin concentrations were shown to up-regulate mRNA levels for VEGF-B and VEGF-R1, suggesting an autocrine/paracrine role for VEGF-B. Higher thrombin concentrations also up-regulated mRNA for VEGF-A and neuropilin-1. In conclusion, the association of MBCP® with thrombin and fibrinogen appears to be a convenient scaffold for bone cell differentiation. Thrombin could also acts at the cellular level by increasing the angiogenic potential of osteoblasts as well as their responsiveness to thrombin and VEGF.

Keywords
Fibrin glue; Osteoblast; Thrombin; Differentiation; VEGF; Biphasic calcium phosphate
First Page Preview
The modulation of gene expression in osteoblasts by thrombin coated on biphasic calcium phosphate ceramic
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 15, May 2006, Pages 2934–2943
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us