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Influence of polymer membrane porosity on C3A hepatoblastoma cell adhesive interaction and function

Paper ID Volume ID Publish Year Pages File Format Full-Text
11747 757 2004 10 PDF Available
Title
Influence of polymer membrane porosity on C3A hepatoblastoma cell adhesive interaction and function
Abstract

The effect of the porosity of acrylonitrile-N-vinylpyrrolidone copolymer membranes on human C3A hepatoblastoma cell adhesive interaction and functioning is investigated on four membranes with an average pore size ranging between 6 and 12 nm. Adhesion of C3A cells was quantified and characterized by studying overall cell morphology and focal adhesion formation. Cell–cell interactions were characterized by E-cadherin expression and organization. Cell growth, fibronectin synthesis and cytochrome P450 activity were estimated as criteria of functional cell activity. The results suggest that membrane porosity influences the initial cell–surface interactions since an increasing pore size augmented cell adhesion and aggregate formation. Cell growth after 7 d was diminished on membranes with an average pore size of 12 nm. The activity of P450 measured by 7-ethoxycoumarin conversion at day 7 was influenced by membrane topography representing a clear optimum in the range of 7–10 nm pore size. These results indicate that membrane porosity is a determinant for the function of hepatocytes in extracorporal liver assist devices.

Keywords
Membrane porosity; Hepatoblastoma cell; (Focal) Adhesion; Cytochrome P450; Cell–surface interaction; Acrylonitrile copolymer
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Influence of polymer membrane porosity on C3A hepatoblastoma cell adhesive interaction and function
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 13, June 2004, Pages 2467–2476
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us