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Biocompatible nanocrystalline octacalcium phosphate thin films obtained by pulsed laser deposition

Paper ID Volume ID Publish Year Pages File Format Full-Text
11755 757 2004 7 PDF Available
Title
Biocompatible nanocrystalline octacalcium phosphate thin films obtained by pulsed laser deposition
Abstract

We extended for the first time pulsed laser ablation to the deposition of octacalcium phosphate Ca8H2(PO4)6·5H2O (OCP) thin films. The depositions were performed with a pulsed UV laser source (λ=248 nm, τ⩾20 ns) in a flux of hot water vapors. The targets were sintered from crystalline OCP powder and the laser ablation fluence was set at values of 1.5–2 J/cm2. During depositions the collectors, Si or Ti substrates, were maintained at a constant temperature within the range 20–200°C. The resulting structures were submitted to heat treatment in hot water vapors for up to 6 h. The best results were obtained at a substrate temperature of 150°C during both deposition and post-deposition treatment. High-resolution electron microscopy and XRD at grazing incidence indicated that the coatings obtained were made of nanocrystalline OCP. Cross-section TEM investigations showed that the coatings contained droplets stacked on Ti substrates as well as distributed across the entire thickness of the arborescence-like structure layers. The results of WST-1 assay, cell adherence, DNA replication, and caspase-1 activity confirmed the good biocompatibility of the coatings.

Keywords
Calcium phosphate coating; Octacalcium phosphate; Laser ablation; TEM; XRD; In vitro test
First Page Preview
Biocompatible nanocrystalline octacalcium phosphate thin films obtained by pulsed laser deposition
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 13, June 2004, Pages 2539–2545
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering