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The role of the CaCl2–guluronic acid interaction on alginate encapsulated βTC3 cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
11762 757 2004 8 PDF Available
Title
The role of the CaCl2–guluronic acid interaction on alginate encapsulated βTC3 cells
Abstract

Previously we demonstrated that alginate composition has a significant effect on the growth of encapsulated βTC3 cells and consequently on the overall metabolic and secretory activities of the encapsulated cultures. Based on these results we postulated that the mechanical properties of alginate were not responsible for the observed effects but rather, changes in the strength of the alginate gel network caused by changes in the number of alginate strands held together in the “egg-box” model are responsible for the observed effects. In this study we address this hypothesis with a series of experiments in which the strength of this interaction is manipulated by varying the calcium concentration either at the time of gelation or during culture maintenance. Our data show that increasing the concentration of the CaCl2 solution used at the time of gelation, thus increasing the strength of the alginate gel network, impedes the growth characteristics of βTC3 cells encapsulated in a high guluronic acid content alginate. This effect is amplified by maintaining a constant supply of calcium ions during culture thus sustaining the interaction between guluronic acid residues and calcium ions. However, preparations of βTC3 cells encapsulated in an alginate with high mannuronic acid content are not affected by changes in CaCl2 concentration due to the low percentage of consecutive guluronic acid residues. Therefore, the present data show that the strength of the alginate gel network is an important factor that influences the growth characteristics of encapsulated cell preparations.

Keywords
Alginate; Calcium; βTC3 insulinoma cells; Bioartificial pancreas
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 25, Issue 13, June 2004, Pages 2603–2610
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us