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Fabrication of collagen-coated biodegradable polymer nanofiber mesh and its potential for endothelial cells growth

Paper ID Volume ID Publish Year Pages File Format Full-Text
11783 758 2005 10 PDF Available
Title
Fabrication of collagen-coated biodegradable polymer nanofiber mesh and its potential for endothelial cells growth
Abstract

Endothelialization of biomaterials is a promising way to prevent intimal hyperplasia of small-diameter vascular grafts. The aim of this study was to design a nanofiber mesh (NFM) that facilitates viability, attachment and phenotypic maintenance of human coronary artery endothelial cells (HCAECs). Collagen-coated poly(l-lactic acid)-co-poly(ε-caprolactone) P(LLA-CL 70:30) NFM with a porosity of 64–67% and a fiber diameter of 470±130 nm was fabricated using electrospinning followed by plasma treatment and collagen coating. The structure of the NFM was observed by SEM and TEM, and mechanical property was studied by tensile test. The presence of collagen on the P(LLA-CL) NFM surface was verified by X-ray photoelectron spectroscopy (XPS) and quantified by colorimetric method. Spatial distribution of the collagen in the NFM was visualized by labelling with fluorescent probe. The collagen-coated P(LLA-CL) NFM enhanced the spreading, viability and attachment of HCAECs, and moreover, preserve HCAEC's phenotype. The P(LLA-CL) NFM is a potential material for tissue engineered vascular graft.

Keywords
Nanofiber; PLLA; PCL; Collagen; Coating; Endothelial cells; Tissue engineering; Vascular grafts
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Fabrication of collagen-coated biodegradable polymer nanofiber mesh and its potential for endothelial cells growth
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 36, December 2005, Pages 7606–7615
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us