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Direct patterning of mammalian cells onto porous tissue engineering substrates using agarose stamps

Paper ID Volume ID Publish Year Pages File Format Full-Text
11786 758 2005 6 PDF Available
Title
Direct patterning of mammalian cells onto porous tissue engineering substrates using agarose stamps
Abstract

This paper describes simple, inexpensive, and potentially generic methodology for generating patterns of mammalian cells on porous scaffolds for tissue engineering using replica printing. Circular patterns (diameter: 200, 700, and 1000 μm) of human osteoblasts were transferred directly from topographically patterned agarose stamps onto porous hydroxyapatite scaffolds or onto fibronectin-coated glass slides. The use of hydrogel stamps provided a “wet”, biocompatible surface and maintained the viability of cells adsorbed on stamps during the patterning process. Stamps inked once with suspensions of cells allowed the repeated patterning of substrates. Direct stamping of human osteoblasts (and, potentially other mammalian cells) can be used to control the size, spacing, and geometry of patterns of cells printed on porous tissue engineering substrates. This approach may find use in controlling the spatial invasion of scaffolds, promoting the hierarchical organization of cells, and in controlling cell–cell interactions as a step in preservation of phenotypes of cells.

Keywords
Hydroxyapatite; Cell printing; Osteoblast
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Direct patterning of mammalian cells onto porous tissue engineering substrates using agarose stamps
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 36, December 2005, Pages 7636–7641
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us