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Atelocollagen for culture of human nucleus pulposus cells forming nucleus pulposus-like tissue in vitro: Influence on the proliferation and proteoglycan production of HNPSV-1 cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
11797 759 2006 8 PDF Available
Title
Atelocollagen for culture of human nucleus pulposus cells forming nucleus pulposus-like tissue in vitro: Influence on the proliferation and proteoglycan production of HNPSV-1 cells
Abstract

Nucleus pulposus (NP) is responsible for maintaining function and structure of the disc. Scaffolds to culture disc cells three-dimensionally are emphasized in recent reports on development of a new method for treating disc degeneration using cell transplantation and tissue engineering. Among artificial scaffolds and cell carrying materials, Atelocollagen is a collagen gel that has an advantage in safety issues over others. However, to date there has been no study that investigated culture of human nucleus pulposus cells in Atelocollagen. To investigate whether Atelocollagen could be used as a culture scaffold and if it has any effect on cell proliferation and proteoglycan (PG) production, as well as to find the optimal commercially available Atelocollagen for NP cell transplantation and tissue engineering, we cultured human NP cell line HNPSV-1, in three different Atelocollagen and compared with alginate. Furthermore, NP-like tissues were generated using these cells and different Atellocollagen solutions. Results showed that both DNA synthesis and content is significantly greater when cultured in Atelocollagen than in alginate. On the other hand, proteoglycan synthesis and accumulation was significantly greater in alginate compared with the 0.3% Atelocollagen scaffolds; with 3% Atelocollagen, however, results were similar. NP-like tissue generated by Atelocollagen showed good water and proteoglycan preservation. The current study demonstrates that the use of Atelocollagen as an in vitro culture scaffold for three-dimensional culture of human NP cell lines is indeed feasible and moreover, Atelocollagen possesses the potential to become a candidate scaffold for cell transplantation or tissue engineering for the treatment of intervertebral disc degeneration.

Keywords
Intervertebral disc; Disc degeneration; Nucleus pulposus; Atelocollagen; Tissue engineering; Scaffold
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Atelocollagen for culture of human nucleus pulposus cells forming nucleus pulposus-like tissue in vitro: Influence on the proliferation and proteoglycan production of HNPSV-1 cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 3, January 2006, Pages 346–353
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us