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Multiple-channel scaffolds to promote spinal cord axon regeneration

Paper ID Volume ID Publish Year Pages File Format Full-Text
11805 759 2006 11 PDF Available
Title
Multiple-channel scaffolds to promote spinal cord axon regeneration
Abstract

As molecular, cellular, and tissue-level treatments for spinal cord injury are discovered, it is likely that combinations of such treatments will be necessary to elicit functional recovery in animal models or patients. We describe multiple-channel, biodegradable scaffolds that serve as the basis for a model to investigate simultaneously the effects on axon regeneration of scaffold architecture, transplanted cells, and locally delivered molecular agents. Poly(lactic-co-glycolic acid) (PLGA) with copolymer ratio 85:15 was used for these initial experiments. Injection molding with rapid solvent evaporation resulted in scaffolds with a plurality of distinct channels running parallel along the length of the scaffolds. The feasibility of creating scaffolds with various channel sizes and geometries was demonstrated. Walls separating open channels were found to possess void fractions as high as 89%, with accessible void fractions as high as 90% through connections 220 μm or larger. Scaffolds degraded in vitro over a period of 30 weeks, over which time-sustained delivery of a surrogate drug was observed for 12 weeks. Primary neonatal Schwann cells were distributed in the channels of the scaffold and remained viable in tissue culture for at least 48 h. Schwann-cell containing scaffolds implanted into transected adult rat spinal cords contained regenerating axons at one month post-operation. Axon regeneration was demonstrated by three-dimensional reconstruction of serial histological sections.

Keywords
Nerve tissue engineering; Scaffold; Microstructure; Schwann cell; Drug release; Image analysis
First Page Preview
Multiple-channel scaffolds to promote spinal cord axon regeneration
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 3, January 2006, Pages 419–429
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering