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Study on the effects of nylon–chitosan-blended membranes on the spheroid-forming activity of human melanocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
11824 760 2006 10 PDF Available
Title
Study on the effects of nylon–chitosan-blended membranes on the spheroid-forming activity of human melanocytes
Abstract

Though reported limitedly in tissue engineering, modification of cellular functions can be achieved by culturing them into multicellular spheroids. We have shown melanocytes form spheroids on chitosan surface. However, how biomaterials promote spheroid formation has never been systemically investigated. In this work, nylon, which inhibits melanocyte spheroid formation, and chitosan, which promotes melanocyte spheroid formation, are used to prepare nylon/chitosan-blended membranes. Membranes composed of pure nylon, pure chitosan and various ratios of nylon and chitosan are employed to examine their effects on spheroid formation. Melanocytes show better adhesion to nylon membranes than that to chitosan membranes. In blended membranes, as more nylon is incorporated, cell adhesion increases and the trend for spheroid formation decreases. Melanocytes can only form spheroids on membranes with poorer cell adhesion. Examining the surface of the blended membranes shows phase separation of nylon and chitosan. As nylon content increases, the nylon phase on the membrane surface increases and thereby enhances cell adhesion. The opposite trend for cell adhesion and spheroid formation substantiates our hypothesis of spheroid formation on biomaterials: a balance between cell–substrate interaction and cell–cell interaction. The decrease in cell–substrate interaction tilts the balance to a state more favorable for spheroid formation. Our work can serve as a model to investigate the relative strengths of cell–cell and cell–substrate interactions and also pave way to design blended membranes with desired physical properties while preserving the spheroid-forming activity.

Keywords
Melanocyte; Chitosan; Nylon; Blend; Spheroid; Vitiligo
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Study on the effects of nylon–chitosan-blended membranes on the spheroid-forming activity of human melanocytes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 29, October 2006, Pages 5079–5088
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us