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Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
11838 761 2006 11 PDF Available
Title
Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications
Abstract

Polyaniline (PANi), a conductive polymer, was blended with a natural protein, gelatin, and co-electrospun into nanofibers to investigate the potential application of such a blend as conductive scaffold for tissue engineering purposes. Electrospun PANi–contained gelatin fibers were characterized using scanning electron microscopy (SEM), electrical conductivity measurement, mechanical tensile testing, and differential scanning calorimetry (DSC). SEM analysis of the blend fibers containing less than 3% PANi in total weight, revealed uniform fibers with no evidence for phase segregation, as also confirmed by DSC. Our data indicate that with increasing the amount of PANi (from 0 to ∼5% w/w), the average fiber size was reduced from 803±121 nm to 61±13 nm (p<0.01p<0.01) and the tensile modulus increased from 499±207 MPa to 1384±105 MPa (p<0.05p<0.05). The results of the DSC study further strengthen our notion that the doping of gelatin with a few % PANi leads to an alteration of the physicochemical properties of gelatin. To test the usefulness of PANi-gelatin blends as a fibrous matrix for supporting cell growth, H9c2 rat cardiac myoblast cells were cultured on fiber-coated glass cover slips. Cell cultures were evaluated in terms of cell proliferation and morphology. Our results indicate that all PANi-gelatin blend fibers supported H9c2 cell attachment and proliferation to a similar degree as the control tissue culture-treated plastic (TCP) and smooth glass substrates. Depending on the concentrations of PANi, the cells initially displayed different morphologies on the fibrous substrates, but after 1week all cultures reached confluence of similar densities and morphology. Taken together these results suggest that PANi-gelatin blend nanofibers might provide a novel conductive material well suited as biocompatible scaffolds for tissue engineering.

Keywords
Electrospinning; Polyaniline (PANi); Gelatin; Tissue engineering; H9c2 cardiac myoblastsCPD, critical point drying; CPSA, camphorsulfonic acid; DMEM, Dulbecco's modified Eagle's medium; DSC, differential scanning calorimetry; EDC, 1-ethyl-3-[3-dimethylami
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 13, May 2006, Pages 2705–2715
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us