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Catalytic efficiency of a thrombomodulin-functionalized membrane-mimetic film in a flow model

Paper ID Volume ID Publish Year Pages File Format Full-Text
11846 761 2006 8 PDF Available
Title
Catalytic efficiency of a thrombomodulin-functionalized membrane-mimetic film in a flow model
Abstract

The protein C anticoagulant pathway generates an “on demand” physiologic anticoagulant response, which is initiated when thrombin binds to thrombomodulin (TM), a transmembrane protein constitutively expressed by endothelial cells. A stable, protein C activating membrane-mimetic film was produced on a polyelectrolyte multilayer (PEM) by in situ photopolymerization of a phospholipid assembly containing TM. The monoacrylated phospholipid monomer was initially synthesized and prepared as unilamellar vesicles with varying molar concentrations of TM. Membrane-mimetic films were constructed on planar substrates with defined surface concentrations of catalytically active TM. 125I-labeled radiolabeling demonstrated little change in TM surface concentration over periods of up to 4 weeks. We utilized a parallel plate flow system to investigate the effects of simulated arterial (500 s−1) and venous (50 s−1) shear rates and TM surface concentration (0–1400 fmol cm−2) on the rate and extent of activation of protein C. The rate of production of activated protein C increased with shear rate and TM surface content. However, in agreement with an analysis of reaction kinetics and mass transfer, experimental results demonstrate that reaction rates become saturated at TM surface densities greater than or equal to 800 fmol cm−2. We believe that the design of membrane-mimetic films that have the capacity to activate the protein C pathway will provide a useful strategy for generating “actively” antithrombogenic surfaces.

Keywords
Anticoagulant; Biomimetics; Thrombomodulin; Anti-thrombogenic; Membrane-mimetic
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Catalytic efficiency of a thrombomodulin-functionalized membrane-mimetic film in a flow model
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 13, May 2006, Pages 2768–2775
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us