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Electrochemical processing of fibrinogen modified-graphite surfaces: Effect on plasmin generation from adsorbed plasminogen

Paper ID Volume ID Publish Year Pages File Format Full-Text
11855 762 2006 9 PDF Available
Title
Electrochemical processing of fibrinogen modified-graphite surfaces: Effect on plasmin generation from adsorbed plasminogen
Abstract

With the aim to improve the fibrinolytic properties of carbons by different biological and electrochemical treatments, we modified graphite surfaces by fibrinogen adsorption and subsequent application of various constant potentials before submitting them to plasminogen adsorption. First, we verified that plasminogen (purified or present in human plasma) could adsorb onto these modified surfaces and that adsorbed plasminogen could be converted by t-PA (the principal physiological activator of plasminogen) to adsorbed plasmin. The catalytic properties of the generated enzyme were characterized in assay solutions containing t-PA, fibrinogen and the chromogenic substrate S-2403 (pyroGlu-Phe-Lys-p-nitroaniline, HCl). Experiments showed that the application of electrical potentials to the fibrinogen coating could indirectly affect the properties of the material. In the case of anodic potentials, the amidolytic activity of the generated plasmin was significantly enhanced. Especially, this activity was 10 times higher at a particular potential value.

Keywords
Carbon; Fibrinogen; Plasminogen; Surface modification; Electrochemistry
First Page Preview
Electrochemical processing of fibrinogen modified-graphite surfaces: Effect on plasmin generation from adsorbed plasminogen
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 1, January 2006, Pages 52–60
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering