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Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide

Paper ID Volume ID Publish Year Pages File Format Full-Text
11859 762 2006 9 PDF Available
Title
Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide
Abstract

Human adipose derived adult stem (hADAS) cells have the ability to differentiate into a chondrogenic phenotype in three-dimensional culture and media containing dexamethasone and TGF-β. The current study examined the potential of a genetically engineered elastin-like polypeptide (ELP) to promote the chondrocytic differentiation of hADAS cells without exogenous chondrogenic supplements. hADAS cells were cultured in ELP hydrogels in either chondrogenic or standard medium at 5% O2 for up to 2 weeks. By day 14, constructs cultured in either medium exhibited significant increases in sulfated glycosaminoglycan (up to 100%) and collagen contents (up to 420%). Immunolabeling confirmed that the matrix formed consisted mainly of type II and not type I collagen. The composition of the constructs cultured in either medium did not differ significantly. To assess the effect of oxygen tension on the differentiation of the above constructs, samples were cultured in standard medium at either 5% or 20% O2 for 7 days and their gene expression profile was evaluated using real time RT-PCR. In both cases, the hADAS–ELP constructs upregulated SOX9 and type II collagen gene expression, while type I collagen was downregulated. However, constructs cultured in 20% O2 highly upregulated type X collagen, which was not detected in the 5% O2 cultures. The study suggests that ELP can promote chondrogenesis for hADAS cells in the absence of exogenous TGF-β1 and dexamethasone, especially under low oxygen tension conditions.

Keywords
Stem cells; Biomaterials; Tissue engineering; Cartilage; Elastin-like polypeptide; ScaffoldhADAS cells, Human adipose-derived adult stem cells; MSC, Bone marrow-derived mesenchymal stem cells; ELP, Elastin-like polypeptide; TGF-β1, Transforming growth fac
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Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 1, January 2006, Pages 91–99
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us