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Immobilization of hemoglobin at the galleries of layered niobate HCa2Nb3O10

Paper ID Volume ID Publish Year Pages File Format Full-Text
11866 764 2005 9 PDF Available
Title
Immobilization of hemoglobin at the galleries of layered niobate HCa2Nb3O10
Abstract

Hemoglobin (Hb) was intercalated at the galleries of layered niobate HCa2Nb3O10 (HCNO). Two different kinds of layered phases of Hb-CNO composites Hb-CNO-1 and Hb-CNO-2 were obtained with the interlayer distances of 7.2 and 10.3 nm in correspondence with the monolayer and bilayer arrangements of proteins between the niobate layers, respectively, based on the powder XRD pattern, HRTEM, UV-vis spectra and CHN analyses. FTIR spectra of Hb-CNO composites show that amide I and amide II bands were actually the same as those of the native Hb, which indicates that there is almost no structural change after immobilization. Michaelis–Menten model methods were used to study the peroxidatic activity of the reaction of 2-methoxyphenol and H2O2 for the entrapped Hb in the galleries of HCNO. Compared to that of free Hb, the kinetic parameters of Hb-CNO kcatkcat, KMKM and kcat/KMkcat/KM were affected by the immobilization process. The immobilized Hb showed a higher relative activity than that of free Hb after incubated in phosphate buffer (pH=7) at 80 °C for a period of time. The environments between the layers of HCNO are hydrophilic which will bind water tightly and help to stabilize the ‘essential water’ layer around the protein. So, immobilization of Hb between the layers of HCNO enhanced the activity of Hb in water-DMSO mixture.

Keywords
Enzyme; Composite; Bioactivity
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Immobilization of hemoglobin at the galleries of layered niobate HCa2Nb3O10
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 26, September 2005, Pages 5267–5275
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us