fulltext.study @t Gmail

The effect of natural extracellular matrix deposited on synthetic polymers on cultured primary hepatocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
11885 765 2006 10 PDF Available
Title
The effect of natural extracellular matrix deposited on synthetic polymers on cultured primary hepatocytes
Abstract

It is well known that natural extracellular matrix (ECM) molecules are deposited on the surface of biomaterials during culture of cells and affect cellular behaviors. However, it has not been fully understood what kinds of ECM molecules are deposited on the surface of biomaterials although the cellular behaviors were affected by deposited ECM. In this study, to investigate the effect of deposited natural ECM on behaviors of hepatocytes cultured on biomaterials such as poly (N-p-vinylbenzyl-4-O-β-d-galactopyranosyl-d-gluconamide) (PVLA) as a hepatocyte-specific matrix and poly (l-lysine) (PLL) as a non-specific one during the culture of hepatocytes in vitro, we investigated expression pattern of ECM genes and adsorption of ECM molecules onto PVLA- and PLL-coated surfaces. It was found that the expression levels of type I collagen and fibronectin genes in the hepatocytes cultured on PVLA-coated surface were different from them in the hepatocytes cultured on PLL-coated one. Also, the results showed that laminin was dominantly deposited on PVLA-coated surface whereas fibronectin was dominantly deposited on PLL-coated one. Hepatocytes maintained liver-specific functions on PVLA- and laminin-coated surfaces. It is thought that deposited laminin during the culture of hepatocytes affects the liver-specific functions of hepatocytes cultured on PVLA-coated surface.

Keywords
Hepatocyte; Extracellular matrix; Laminin; Fibronectin; Adsorption
First Page Preview
The effect of natural extracellular matrix deposited on synthetic polymers on cultured primary hepatocytes
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 26, September 2006, Pages 4519–4528
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering