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In situ observation of biomolecules patterned on a PEG-modified Si surface by scanning probe lithography

Paper ID Volume ID Publish Year Pages File Format Full-Text
11900 765 2006 6 PDF Available
Title
In situ observation of biomolecules patterned on a PEG-modified Si surface by scanning probe lithography
Abstract

A Si(1 0 0) wafer was modified with methoxy-poly(ethylene glycol) (M-PEG silane) via a self-assembly technique and nano-/micro-sized patterns were then fabricated by scanning probe lithography. The protrusive silicon dioxide pattern was more reactive compared to the non-patterned area, i.e. the PEG deposited area. To demonstrate the feasibility of the submicron patterning of protein based on the anodic oxidation of the Si surface by atomic force microscopy (AFM), streptavidin labelled with Au-colloidal particle and non-labelled streptavidin were site-selectively immobilized on the patterned areas. The streptavidin-patterned surface produced by these procedures can be utilized for the detection of biotinylated materials, such as an antibody and an antigen. A patterned silicon surface is the basis of biosensing devices, in which the patterned areas serve as sensing elements that directly interact with bioanalytes, while the background of the substrate remains passive to the deposition of analytes, thus resulting in a high signal-to-noise ratio.

Keywords
Atomic force microscopy (AFM); Self-assembly; Scanning probe lithography (SPL); Surface modification; Patterning; Protein adsorption
First Page Preview
In situ observation of biomolecules patterned on a PEG-modified Si surface by scanning probe lithography
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 26, September 2006, Pages 4655–4660
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering