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Surface study of collagen/poloxamine hydrogels by a ‘deep freezing’ ToF-SIMS approach

Paper ID Volume ID Publish Year Pages File Format Full-Text
11904 766 2006 9 PDF Available
Title
Surface study of collagen/poloxamine hydrogels by a ‘deep freezing’ ToF-SIMS approach
Abstract

In order to determine the presence of collagen molecules at the surface of a collagen-modified poloxamine hydrogel (a semi-interpenetrating network), the surface composition was studied using Time-of-Flight Secondary Ion Mass Spectra (ToF-SIMS). Collagen was added to the poloxamine hydrogel (poloxamine is a commercially available four-arm poly(ethylene oxide)/poly(propylene oxide) block copolymer, PEO/PPO) to promote the attachment of endothelial or liver cells. X-ray photoelectron spectroscopy (XPS) of dry samples showed a sharp increase in the N content from 0.6% in a pure poloxamine hydrogel to 8.8% in the collagen-containing material. Afterwards, the surface was studied by a ‘deep freezing’ ToF-SIMS approach under progressive heating from −120 to −60 °C. The positive spectrum of collagen/poloxamine at −65 °C displayed distinct signals corresponding to different amino acid fragments such as CH4N+ (30 m/zm/z, Gly), C3HN2+ (43 m/zm/z, Arg), C2H6N+ (44 m/zm/z, Ala) and C4H5N2+ (81 m/zm/z, His) and others corresponding to the PEO and PPO blocks of poloxamine. In addition, the negative spectrum showed peaks at 26 m/zm/z (CN−), 32 m/zm/z (S−) and 42 m/zm/z (CNO−) characteristic of fragments of the collagen molecule. Imaging experiments indicated the homogeneous distribution of the collagen on the surface. These results supported the use of ToF-SIMS for the surface characterization of hydrated hydrogels and confirmed the collagen presence as the means whereby cells attach to the modified poloxamine matrix.

Keywords
Surface analysis; ‘Deep freezing’ ToF-SIMS; Hydrogels; Collagen; Poloxamine
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Surface study of collagen/poloxamine hydrogels by a ‘deep freezing’ ToF-SIMS approach
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 11, April 2006, Pages 2340–2348
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us