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Combined microscale mechanical topography and chemical patterns on polymer cell culture substrates

Paper ID Volume ID Publish Year Pages File Format Full-Text
11919 766 2006 8 PDF Available
Title
Combined microscale mechanical topography and chemical patterns on polymer cell culture substrates
Abstract

This paper presents a technique to independently form mechanical topography and surface chemical patterns on polymer cell substrates, and studies the response of osteoblast cells to these surface patterns. The patterns were formed in two separate steps: hot embossing imprint lithography formed the mechanical topography and microcontact printing created the chemical pattern. The resulting substrate had surface features consisting of embossed grooves 4 μm deep and 8 μm wide spaced by 16 μm wide mesas and microcontact printed adhesive lanes 10 μm wide with spacings that ranged from 10 to 100 μm. When presented with either mechanical topography or chemical patterns alone, the cells significantly aligned to the pattern presented. When presented with mechanical topography overlaid with an orthogonal chemical pattern, the cells aligned to the mechanical topography. As the chemical pattern spacing was increased, osteoblasts remained aligned to the mechanical topography. Unlike traditional microfabrication approaches based on photolithography and wet chemistry, the patterning technique presented is compatible with a large number of biomaterials, could form patterns with features much smaller than 1 μm, and is highly scalable to large substrates.

Keywords
Micropatterning; Surface topography; Chemical patterns; Alignment; Adhesion
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Combined microscale mechanical topography and chemical patterns on polymer cell culture substrates
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 11, April 2006, Pages 2487–2494
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us