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Development of mammalian cell-enclosing subsieve-size agarose capsules (<100 μm) for cell therapy

Paper ID Volume ID Publish Year Pages File Format Full-Text
11953 769 2005 7 PDF Available
Title
Development of mammalian cell-enclosing subsieve-size agarose capsules (<100 μm) for cell therapy
Abstract

Agarose capsules were prepared using a droplet breakup method in a coflowing stream. Subsieve-size capsules 76±9 μm in diameter were obtained by extruding 4 wt% agarose solution from a needle (300 μm inner diameter) at a velocity of 1.2 cm/s into an ambient liquid paraffin flow of 20.8 cm/s. Increasing the flow rate of the liquid paraffin and decreasing that of the agarose solution resulted in a decreased resultant capsule diameter. Reduction in diameter from several hundred micrometers to subsieve-size (<100 μm) enhanced molecular exchange and mechanical stability. Measurements based on the percentage of intact mitochondria in the cells demonstrated that the viability of the enclosed cells was independent of capsule diameter. No significant difference was observed between the viabilities of cells enclosed in capsules with diameters of 79±8 and 351±41 μm (p=0.43p=0.43). Compared with cells seeded in a tissue culture dish, the cells enclosed in the subsieve-size capsules showed 89.2% viability.

Keywords
Microcapsule; Cell encapsulation; Microencapsulation; Transplantation; Polysaccharide; Cell viability
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Development of mammalian cell-enclosing subsieve-size agarose capsules (<100 μm) for cell therapy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 23, August 2005, Pages 4786–4792
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us