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The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
11959 769 2005 9 PDF Available
Title
The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture
Abstract

Our research group aims to develop an osteochondral composite using type II collagen gel with hydroxyapatite (HAp) deposited on one side. Soaking gels in Ca2+ and phosphate solution is indispensable to HAp deposition, so relationships between cell behavior and Ca2+ concentration were examined in two- and three-dimensional cultures. The present results indicate that 2–4 mM Ca2+ is suitable for proliferation and survival of osteoblasts, whereas slightly higher concentrations (6–8 mM) favor osteoblast differentiation and matrix mineralization in both 2- and 3-dimensional cultures. Higher concentrations (>10mM) are cytotoxic. Purely from the perspective of calcium deposition, higher concentrations lead to increased accumulation of Ca2+.Culturing cells in phosphate-containing gel in media with Ca2+ also leads to time-dependent formation of HAp in the gel. Considering the viability of embedded cells, culturing scaffolds in media with Ca2+ concentrations around 5 mM is useful for both HAp deposition and osteoblast behavior.

Keywords
Calcification; Medium calcium; Osteoblast; Viability; Type II collagen gel; Tissue engineering
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The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 26, Issue 23, August 2005, Pages 4847–4855
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us