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Stem cell attachment to layer-by-layer assembled TiO2 nanoparticle thin films

Paper ID Volume ID Publish Year Pages File Format Full-Text
11970 770 2006 8 PDF Available
Title
Stem cell attachment to layer-by-layer assembled TiO2 nanoparticle thin films
Abstract

Surface topography is one of the most important factors influencing the attachment and spreading of cells. In the present study, layer-by-layer assembled titanium dioxide (TiO2) nanoparticle thin films were chosen for attachment, proliferation and spreading studies on mouse mesenchymal stem cells (MSC). Increasing surface roughness was observed with increasing number of layer-by-layer assembled TiO2 thin films. Four layer TiO2 thin film showed higher number of attached cells than a one layer thin film and control surfaces. MSCs experienced no cytotoxic effects after culture on the TiO2 coated substrates as observed from the cytotoxicity tests. Cell spreading, visualized with scanning electron microscopy, showed a faster rate of spreading on a rougher surface. Cells on a four-layer substrate, at 12 h showed complete spreading, where as most of the cells on a control surface and a one-layer surface, at 24 h, retained a rounded morphology. In conclusion, TiO2 nanoparticle thin films were successfully assembled in alternation with polyelectrolytes and in-vitro studies with MSC showed an increase in the attachment and faster spreading of cells on rougher surfaces.

Keywords
Layer-by-layer assembly; Stem cells; Nanoparticles; Surface roughness; Surface modification; Titanium dioxide
First Page Preview
Stem cell attachment to layer-by-layer assembled TiO2 nanoparticle thin films
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 24, August 2006, Pages 4296–4303
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering