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Autoactivation of blood factor XII at hydrophilic and hydrophobic surfaces ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
11973 770 2006 8 PDF Available
Title
Autoactivation of blood factor XII at hydrophilic and hydrophobic surfaces ☆
Abstract

Contact activation of blood factor XII (FXII, Hageman factor) in neat-buffer solution is shown not to be specific for anionic hydrophilic procoagulants as proposed by the accepted biochemistry of surface activation. Rather, FXII activation in the presence of plasma proteins leads to an apparent specificity   for hydrophilic surfaces that is actually due to a relative diminution of the FXII→FXIIa reaction at hydrophobic surfaces. FXII activation in neat-buffer solution was effectively instantaneous upon contact with either hydrophilic (fully water-wettable clean glass) or hydrophobic (poorly water-wettable silanized glass) procoagulant particles, with greater FXIIa yield obtained by activation with hydrophobic procoagulants. In sharp contrast, both activation rate and yield was found to be significantly attenuated at hydrophobic surfaces in the presence of plasma proteins. Putative FXIIa produced by surface activation with both hydrophilic and hydrophobic procoagulants was shown to hydrolyze blood factor XI (FXI) to the activated form FXIa (FXI⟶FXIIaFXIa) that causes FXI-deficient plasma to rapidly coagulate.

Keywords
Blood coagulation; FXII; Hageman factor; Contact activation; Autoactivation
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Autoactivation of blood factor XII at hydrophilic and hydrophobic surfaces ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 27, Issue 24, August 2006, Pages 4325–4332
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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Full-text PDF Download
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